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Research Article

Whole Genome Sequencing versus Traditional Genotyping for Investigation of a Mycobacterium tuberculosis Outbreak: A Longitudinal Molecular Epidemiological Study

  • Andreas Roetzer equal contributor,

    equal contributor Contributed equally to this work with: Andreas Roetzer, Roland Diel, Thomas A. Kohl

    Affiliation: Molecular Mycobacteriology, Forschungszentrum Borstel, Borstel, Germany

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  • Roland Diel equal contributor,

    equal contributor Contributed equally to this work with: Andreas Roetzer, Roland Diel, Thomas A. Kohl

    Affiliation: Institute for Epidemiology, Schleswig-Holstein University Hospital, Kiel, Germany

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  • Thomas A. Kohl equal contributor,

    equal contributor Contributed equally to this work with: Andreas Roetzer, Roland Diel, Thomas A. Kohl

    Affiliations: Molecular Mycobacteriology, Forschungszentrum Borstel, Borstel, Germany, Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld University, Bielefeld, Germany

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  • Christian Rückert,

    Affiliation: Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld University, Bielefeld, Germany

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  • Ulrich Nübel,

    Affiliation: Robert Koch Institut, Wernigerode, Germany

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  • Jochen Blom,

    Affiliation: Computational Genomics, CeBiTec, Bielefeld University, Bielefeld, Germany

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  • Thierry Wirth,

    Affiliation: Department of Systematics and Evolution, Muséum National d'Histoire Naturelle, École Pratique des Hautes Études, Paris, France

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  • Sebastian Jaenicke,

    Affiliation: Computational Genomics, CeBiTec, Bielefeld University, Bielefeld, Germany

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  • Sieglinde Schuback,

    Affiliation: Gesundheitsamt des Kreises Steinburg, Itzehoe, Germany

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  • Sabine Rüsch-Gerdes,

    Affiliation: National Reference Center for Mycobacteria, Forschungszentrum Borstel, Borstel, Germany

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  • Philip Supply,

    Affiliation: INSERM, U1019, CNRS UMR 8204, Institut Pasteur de Lille, Univ Lille Nord de France, Lille, France

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  • Jörn Kalinowski,

    Affiliation: Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld University, Bielefeld, Germany

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  • Stefan Niemann mail

    sniemann@fz-borstel.de

    Affiliation: Molecular Mycobacteriology, Forschungszentrum Borstel, Borstel, Germany

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  • Published: February 12, 2013
  • DOI: 10.1371/journal.pmed.1001387

About the Authors

Andreas Roetzer, Thomas A. Kohl, Stefan Niemann
Molecular Mycobacteriology, Forschungszentrum Borstel, Borstel, Germany
Roland Diel
Institute for Epidemiology, Schleswig-Holstein University Hospital, Kiel, Germany
Thomas A. Kohl, Christian Rückert, Jörn Kalinowski
Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld University, Bielefeld, Germany
Ulrich Nübel
Robert Koch Institut, Wernigerode, Germany
Jochen Blom, Sebastian Jaenicke
Computational Genomics, CeBiTec, Bielefeld University, Bielefeld, Germany
Thierry Wirth
Department of Systematics and Evolution, Muséum National d'Histoire Naturelle, École Pratique des Hautes Études, Paris, France
Sieglinde Schuback
Gesundheitsamt des Kreises Steinburg, Itzehoe, Germany
Sabine Rüsch-Gerdes
National Reference Center for Mycobacteria, Forschungszentrum Borstel, Borstel, Germany
Philip Supply
INSERM, U1019, CNRS UMR 8204, Institut Pasteur de Lille, Univ Lille Nord de France, Lille, France

Corresponding Author

Email: sniemann@fz-borstel.de

Competing Interests

PS is a consultant for Genoscreen. All other authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: AR TK SN. Performed the experiments: AR TK AR CR JB SN. Analyzed the data: AR RD TK CR UN JB TW SJ SS SR PS JK SN. Contributed reagents/materials/analysis tools: AR RD TK CR UN JB TW SJ SS SR PS JK SN. Wrote the first draft of the manuscript: AR TK SN . Contributed to the writing of the manuscript: AR RD TK CR UN JB TW SJ SS SRG PS JK SN . ICMJE criteria for authorship read and met: AR RD TK CR UN JB TW SJ SS SRG PS JK SN. Agree with manuscript results and conclusions: AR RD TK CR UN JB TW SJ SS SRG PS JK SN.