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Research Article

Antigen Load and Viral Sequence Diversification Determine the Functional Profile of HIV-1–Specific CD8+ T Cells

  • Hendrik Streeck,

    Affiliation: Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Zabrina L Brumme,

    Affiliation: Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Michael Anastario,

    Affiliation: Center for Disaster and Humanitarian Assistance Medicine, Department of Military and Emergency Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America

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  • Kristin W Cohen,

    Affiliation: Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Jonathan S Jolin,

    Affiliation: Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Angela Meier,

    Affiliation: Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Chanson J Brumme,

    Affiliation: Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Eric S Rosenberg,

    Affiliation: Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Galit Alter,

    Affiliation: Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Todd M Allen,

    Affiliation: Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Bruce D Walker,

    Affiliations: Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts, United States of America, Howard Hughes Medical Institute, Chevy Chase, Maryland, United States of America

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  • Marcus Altfeld mail

    To whom correspondence should be addressed. E-mail: maltfeld@partners.org

    Affiliation: Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Published: May 06, 2008
  • DOI: 10.1371/journal.pmed.0050100

About the Authors

Hendrik Streeck, Zabrina L Brumme, Kristin W Cohen, Jonathan S Jolin, Angela Meier, Chanson J Brumme, Eric S Rosenberg, Galit Alter, Todd M Allen, Bruce D Walker, Marcus Altfeld
Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts, United States of America
Michael Anastario
Center for Disaster and Humanitarian Assistance Medicine, Department of Military and Emergency Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America
Bruce D Walker
Howard Hughes Medical Institute, Chevy Chase, Maryland, United States of America

Corresponding Author

Email: maltfeld@partners.org

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

H. Streeck and M. Altfeld designed the experiments/the study. H. Streeck, Z.L. Brumme, K.W. Cohen, J.S. Jolin, and T.M. Allen collected data or did experiments for the study. H. Streeck, Z.L. Brumme, M. Anastario, J.S. Jolin, AM, CJB, and T.M. Allen analyzed the data. H. Streeck wrote the first draft of the paper. H. Streeck, Z.L. Brumme, M. Anastario, A. Meier, C.J. Brumme, G. Alter, B.D. Walker, and M. Altfeld contributed to writing the paper. Z.L. Brumme performed all HIV RNA sequencing and analyzed these data. K.W. Cohen performed some of the intracellular cytokine staining and flow cytometry acquisition. E.S. Rosenberg designed and recruited patient cohort, and enrolled patients. T.M. Allen was responsible for generation of viral sequence data.