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Research Article

Polymorphisms, Mutations, and Amplification of the EGFR Gene in Non-Small Cell Lung Cancers

  • Masaharu Nomura,

    Affiliation: Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America

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  • Hisayuki Shigematsu,

    Affiliation: Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America

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  • Lin Li,

    Affiliation: Cancer Prevention Research, Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

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  • Makoto Suzuki,

    Affiliation: Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America

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  • Takao Takahashi,

    Affiliation: Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America

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  • Pila Estess,

    Affiliation: Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America

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  • Mark Siegelman,

    Affiliation: Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America

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  • Ziding Feng,

    Affiliation: Cancer Prevention Research, Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

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  • Harubumi Kato,

    Affiliation: First Department of Surgery, Tokyo Medical University, Tokyo, Japan

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  • Antonio Marchetti,

    Affiliation: Pathology Unit, Clinical Research Center, Center of Excellence on Aging, University Foundation, Chieti, Italy

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  • Jerry W Shay,

    Affiliation: Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America

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  • Margaret R Spitz,

    Affiliation: Department of Epidemiology, University of Texas M. D. Anderson Cancer Center, Houston, Texas, United States of America

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  • Ignacio I Wistuba,

    Affiliation: Department of Pathology, University of Texas M. D. Anderson Cancer Center, Houston, Texas, United States of America

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  • John D Minna,

    Affiliations: Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America, Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America

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  • Adi F Gazdar mail

    To whom correspondence should be addressed. E-mail: adi.gazdar@utsouthwestern.edu

    Affiliations: Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America, Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America

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  • Published: April 24, 2007
  • DOI: 10.1371/journal.pmed.0040125

About the Authors

Masaharu Nomura, Hisayuki Shigematsu, Makoto Suzuki, Takao Takahashi, John D Minna, Adi F Gazdar
Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
Lin Li, Ziding Feng
Cancer Prevention Research, Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America
Pila Estess, Mark Siegelman, Adi F Gazdar
Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
Harubumi Kato
First Department of Surgery, Tokyo Medical University, Tokyo, Japan
Antonio Marchetti
Pathology Unit, Clinical Research Center, Center of Excellence on Aging, University Foundation, Chieti, Italy
Jerry W Shay
Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
Margaret R Spitz
Department of Epidemiology, University of Texas M. D. Anderson Cancer Center, Houston, Texas, United States of America
Ignacio I Wistuba
Department of Pathology, University of Texas M. D. Anderson Cancer Center, Houston, Texas, United States of America
John D Minna
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
John D Minna
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America

Corresponding Author

Email: adi.gazdar@utsouthwestern.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

M. Nomura, H. Shigematsu, P. Estess, M. Siegelman, and A. F. Gazdar designed the study. M. Nomura, H. Shigematsu, T. Takahashi and I. I. Wistuba collected data or performed experiments for the study. M. Nomura made the primer sets for the target genes and modified the conditions of PCR reactions. M. Suzuki, H. Shigematsu, and I. I. Wistuba collected the samples for the study and their clinicopathological data. A. F. Gazdar supervised the analysis of the data. M. Nomura, L. Li, Z. Feng, H. Kato, J. D. Minna, and A. F. Gazdar analyzed the data. P. Estess interpreted early data, designed subsequent approaches, and provided expertise in experimental approaches. M. Siegelman provided technical expertise and instrumentation to perform the analysis. A. Marchetti analyzed the DNA samples for EGFR mutations. M. Suzuki, H. Shigematsu, A. Marchetti, M. R. Spitz, and I. I. Wistuba enrolled patients. A. Marchetti collected tissues and data from Italian patients in the study and extracted DNA samples from tissues. M. R. Spitz provided the DNA samples from normal individuals in the US. I. I. Wistuba provided the DNA samples from patients in US. J. W. Shay and J. D. Minna provided HBECs. All authors contributed to writing the paper.