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Research Article

Impaired Hepatitis C Virus-Specific T Cell Responses and Recurrent Hepatitis C Virus in HIV Coinfection

  • Arthur Y Kim equal contributor mail,

    equal contributor Contributed equally to this work with: Arthur Y Kim, Julian Schulze zur Wiesch

    To whom correspondence should be addressed. E-mail: akim1@partners.org

    Affiliation: Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Julian Schulze zur Wiesch equal contributor,

    equal contributor Contributed equally to this work with: Arthur Y Kim, Julian Schulze zur Wiesch

    Affiliations: Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America, Howard Hughes Medical Institute, Chevy Chase, Maryland, United States of America

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  • Thomas Kuntzen,

    Affiliation: Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Joerg Timm,

    Affiliation: Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Daniel E Kaufmann,

    Affiliation: Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Jared E Duncan,

    Affiliation: Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Andrea M Jones,

    Affiliation: Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Alysse G Wurcel,

    Affiliation: Lemuel Shattuck Hospital, Jamaica Plain, Massachusetts, United States of America

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  • Benjamin T Davis,

    Affiliation: Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Rajesh T Gandhi,

    Affiliation: Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Gregory K Robbins,

    Affiliation: Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Todd M Allen,

    Affiliation: Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Raymond T Chung,

    Affiliation: Gastrointestinal Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Georg M Lauer,

    Affiliation: Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Bruce D Walker

    Affiliations: Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America, Howard Hughes Medical Institute, Chevy Chase, Maryland, United States of America

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  • Published: December 12, 2006
  • DOI: 10.1371/journal.pmed.0030492

About the Authors

Arthur Y Kim, Julian Schulze zur Wiesch, Thomas Kuntzen, Joerg Timm, Daniel E Kaufmann, Jared E Duncan, Andrea M Jones, Benjamin T Davis, Rajesh T Gandhi, Gregory K Robbins, Todd M Allen, Georg M Lauer, Bruce D Walker
Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America
Julian Schulze zur Wiesch, Bruce D Walker
Howard Hughes Medical Institute, Chevy Chase, Maryland, United States of America
Alysse G Wurcel
Lemuel Shattuck Hospital, Jamaica Plain, Massachusetts, United States of America
Raymond T Chung
Gastrointestinal Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America

Corresponding Author

Email: akim1@partners.org

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

AYK, JSzW, GML, and BDW designed the study. AYK, JSzW, TMA, GML, and BDW analyzed the data. AYK, JSzW, AGW, BTD, RTG, GKR, and RTC enrolled patients. AYK, JSzW, TK, JT, DEK, AMJ, BTD, RTG, GKR, TMA, RTC, GML, and BDW contributed to writing the paper. AYK, JSzW, AMJ, and JED performed the tissue culture work, including but not limited to: blood processing, cell culture, lymphoproliferation assays, elispot analysis, flow cytometry, maintaining cell lines, tetramer analysis, and DNA extractions. JT contributed to the sample processing, lymphoproliferation assays, Elispot assays, and cell culture work. TK and JT designed additional experiments to try to amplify virus from time points without detectable plasma viremia. BTD, RTG, and GKR identified patients with recurrent/relapsed HCV from the Infectious Disease Clinic at Massachusetts General Hospital and in collecting clinical data.