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Research Article

Mycobacterium tuberculosis Induces Interleukin-32 Production through a Caspase- 1/IL-18/Interferon-γ-Dependent Mechanism

  • Mihai G Netea mail,

    To whom correspondence should be addressed. E-mail: M.Netea@aig.umcn.nl

    Affiliations: Division of Infectious Diseases, University of Colorado Health Sciences Center, Denver, Colorado, United States of America, Nijmegen University Center for Infectious Diseases, Nijmegen, Netherlands

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  • Tania Azam,

    Affiliation: Division of Infectious Diseases, University of Colorado Health Sciences Center, Denver, Colorado, United States of America

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  • Eli C Lewis,

    Affiliation: Division of Infectious Diseases, University of Colorado Health Sciences Center, Denver, Colorado, United States of America

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  • Leo A. B Joosten,

    Affiliation: Laboratory of Experimental Rheumatology, Radboud University Nijmegen Medical Center, Nijmegen, Netherlands

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  • Maorong Wang,

    Affiliation: Division of Surgery, University of Colorado Health Sciences Center, Denver, Colorado, United States of America

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  • Dennis Langenberg,

    Affiliation: Department of Immunohematology and Blood Transfusion, Leiden University, Leiden, Netherlands

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  • Xianzhong Meng,

    Affiliation: Division of Surgery, University of Colorado Health Sciences Center, Denver, Colorado, United States of America

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  • Edward D Chan,

    Affiliation: Division of Pulmonary Sciences, University of Colorado Health Sciences Center, Denver, Colorado, United States of America

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  • Do-Young Yoon,

    Affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, Korea

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  • Tom Ottenhoff,

    Affiliation: Department of Immunohematology and Blood Transfusion, Leiden University, Leiden, Netherlands

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  • Soo-Hyun Kim,

    Affiliations: Division of Infectious Diseases, University of Colorado Health Sciences Center, Denver, Colorado, United States of America, Department of Biomedical Science and Technology, Konkuk University, Seoul, Korea

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  • Charles A Dinarello

    Affiliation: Division of Infectious Diseases, University of Colorado Health Sciences Center, Denver, Colorado, United States of America

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  • Published: August 15, 2006
  • DOI: 10.1371/journal.pmed.0030277

About the Authors

Mihai G Netea, Tania Azam, Eli C Lewis, Soo-Hyun Kim, Charles A Dinarello
Division of Infectious Diseases, University of Colorado Health Sciences Center, Denver, Colorado, United States of America
Mihai G Netea
Nijmegen University Center for Infectious Diseases, Nijmegen, Netherlands
Leo A. B Joosten
Laboratory of Experimental Rheumatology, Radboud University Nijmegen Medical Center, Nijmegen, Netherlands
Maorong Wang, Xianzhong Meng
Division of Surgery, University of Colorado Health Sciences Center, Denver, Colorado, United States of America
Dennis Langenberg, Tom Ottenhoff
Department of Immunohematology and Blood Transfusion, Leiden University, Leiden, Netherlands
Edward D Chan
Division of Pulmonary Sciences, University of Colorado Health Sciences Center, Denver, Colorado, United States of America
Do-Young Yoon
Department of Bioscience and Biotechnology, Konkuk University, Seoul, Korea
Soo-Hyun Kim
Department of Biomedical Science and Technology, Konkuk University, Seoul, Korea

Corresponding Author

Email: M.Netea@aig.umcn.nl

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

MGN, DL, TO, SHK, and CAD designed the study. TA, ECL, LABJ, MW, DL, and XM collected data or did experiments for the study. TA helped generate recombinant IL-32. LABJ participated in IL-32 expression of PBMCs after exposure to LPS and MTB. MW participated in the immunocytochemistry for IL-32 staining in PBMCs treated with different stimuli and took the picture. DL provided the necessary mycobacterial stimulating components and help in using them in the experiments. DYY prepared anti-IL-32 antibodies. CAD developed the assays also. EDC participated in intellectual input and discussions with MGN. MGN, XM, TO, SHK, and CAD analyzed the data. MGN, EDC, TO, and CAD contributed to writing the paper.